Tissue Culture and Immune Chemical
Tissue Culture and Immuno Chemical Lab
TCIC lab play essential rule in environmental research, by providing several biosystems can affected by pollutants or by investigation the pollutants effects on these organizims in natural environment, by carrying out direct and indirect toxicity, gentoxicity, immunotoxicity In vivo and In vitro for drugs, foods, water, air, soil and plant extracts, on human peripheral blood lymphocytes, human and mice bone marrow, macrophage and microphage suspension cells, as well as effects of environmental factors on bacterial, fungal, and algal cells suspension by measuring biomass, viability, activity and cell dimensions, in addition to calculate LD50 ,LC50 for different compounds.
Practecally chromosomal aberrations techniques ( solid and banding) beside to human karyotyping are carrying out by depended methodes in the study of genotoxic effects. In addition to dependent standrd methods from WHO, APHA and methods which were published in pre reviwed journals likeJournal of Analytical Toxicology and Mutation Research.
NO. |
TESTS |
1 |
Acute toxicity assay |
2 |
Chronic toxicity assay |
3 |
Lethal and sub lethal dose |
4 |
Total WBC count |
5 |
Deferential WBC count |
6 |
Bleeding and clotting time |
7 |
Hb test |
8 |
PVC test |
9 |
ESR test |
10 |
Albumin concentration test |
11 |
Liver function ALP, ACP, GOT, GPT, TSB |
12 |
Kidney function (Blood Urea, Uric Acid, Creatinine, GUE) |
13 |
Arthus and delayed hyper sensitivity assay |
14 |
phagocytoses assay |
15 |
Heamagglutination test |
16 |
RF, Widal test, RB test, CRP, SOT |
17 |
Total protein test |
18 |
Total lipid test |
19 |
Total sugar test |
20 |
Humidity test |
21 |
Alkaloid, Falvinoid, Tannin, Saponin, Comarin, Phenols, protein, chlorophyll |
22 |
Prepare of standard solution and culture media of above tests |