Tissue Culture and Immune Chemical

Tissue Culture and Immuno Chemical Lab 

TCIC lab play essential rule in environmental research, by providing several biosystems can affected by pollutants or by investigation the pollutants effects on these organizims in natural environment, by carrying out direct and indirect toxicity, gentoxicity, immunotoxicity In vivo and In vitro for drugs, foods, water, air, soil and plant extracts,  on human peripheral blood lymphocytes, human and mice bone marrow, macrophage and microphage suspension cells, as well as effects of environmental factors on bacterial, fungal, and algal cells suspension by measuring biomass, viability, activity and cell dimensions, in addition to calculate LD50 ,LC50  for different compounds.   

Practecally chromosomal aberrations techniques ( solid and banding) beside to human karyotyping are carrying out by depended  methodes in the study of genotoxic effects. In addition to dependent standrd methods from WHO, APHA and methods which were published in pre reviwed journals likeJournal of Analytical Toxicology and Mutation Research.

 

NO.

TESTS

1

Acute toxicity assay

2

Chronic toxicity assay

3

Lethal and sub lethal dose

4

Total WBC count 

5

Deferential  WBC count

6

Bleeding and clotting time

7

Hb test 

8

PVC test

9

ESR test

10

Albumin concentration test

11

Liver function  ALP, ACP, GOT, GPT, TSB

12

Kidney function  (Blood Urea, Uric Acid, Creatinine, GUE)

13

Arthus and delayed hyper sensitivity assay

14

phagocytoses assay

15

Heamagglutination test

16

RF, Widal test,  RB test, CRP, SOT 

17

Total protein test

18

Total lipid test 

19

Total sugar test

20

Humidity test

21

Alkaloid, Falvinoid, Tannin, Saponin,  Comarin,  Phenols, protein, chlorophyll 

22  

Prepare of standard solution and culture media of above tests

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